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CO2 incubator gas path by the high concentration of CO2 cylinders, air pumps, regulator valve, needle valves, solenoid valves, flow meters and gas cylinders and other components. (High concentrations of CO2 cylinders provided by the user). When a certain pressure of high concentration of CO2 gas input, through the control valve, needle valve and solenoid valve flow, time control, to ensure that a certain amount of CO2 gas into the studio, to automatically control the value of CO2 concentration studio. The value at the same time through the display circuit, easy to observe and understand. In order to facilitate the user in the use of concentration monitoring and sampling, CO2 tank in the back with sampling, monitoring port. Due to the use of fast and slow double inflation method, the CO2 tank in the open, close the door, the studio CO2 concentration can be quickly restored and no overshoot phenomenon. During the CO2 tank work, the gas cylinders are used to supplement the working chamber to maintain a stable CO2 concentration value.